Growth factors and cytokines frequently appear together in discussions. They share several structural and functional similarities and both bind to unique cell surface receptors that start signaling pathways and have structurally distinct receptors. Additionally, many of the intracellular signaling elements that the activated cell surface receptor uses to communicate with the cell nucleus are shared. The growth factors strongly impact cell growth, differentiation, survival, inflammation, and tissue repair.

It is undeniable that the usage of pure, high-quality proteins has significant advantages for cell cultures. Cells in culture are given growth factors to create the best conditions for health and development. Some naughty cells require a serum-free medium, which frequently calls for the usage of spec c cytokines and growth factors. For primary cells, this is always true, but it is also commonly required for transformed cell lines and hybridomas. Fro example, EGF is necessities for human skin keratinocyte primary cultures, and EGF and FGFs are all able to take the place of the serum component in the culture media of HeLa cells. To put it another way, the choice of the appropriate cytokines and growth factors for your application is crucial.





 

Ref.

1. Schlessinger J, Schreiber AB, Levi A, Lax I, Libermann T, Yarden Y. Regulation of cell proliferation by epidermal growth factor. CRC Crit Rev Biochem. 1983;14(2):93-111.
2. Tamama K, Fan VH, Griffith LG, Blair HC, Wells A. Epidermal growth factor as a candidate for ex vivo expansion of bone marrow-derived mesenchymal stem cells. Stem Cells. 2006 Mar;24(3):686-95.
3. Miller-Kobisher B, Suárez-Vega DV, Velazco de Maldonado GJ. Epidermal Growth Factor in Aesthetics and Regenerative Medicine: Systematic Review. J Cutan Aesthet Surg. 2021 Apr-Jun;14(2):137-146.
4. Hagl CI, Wink E, Scherf S, Heumüller-Klug S, Hausott B, Schäfer KH. FGF2 deficit during development leads to specific neuronal cell loss in the enteric nervous system. Histochem Cell Biol. 2013 Jan;139(1):47-57.
5. Vashi AV, Abberton KM, Thomas GP, Morrison WA, O'Connor AJ, Cooper-White JJ, Thompson EW. Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix. Tissue Eng. 2006 Nov;12(11):3035-43.
6. Rider DA, Dombrowski C, Sawyer AA, Ng GH, Leong D, Hutmacher DW, Nurcombe V, Cool SM. Autocrine fibroblast growth factor 2 increases the multipotentiality of human adipose-derived mesenchymal stem cells. Stem Cells. 2008 Jun;26(6):1598-608.
7. Schooltink H, Stoyan T, Roeb E, Heinrich PC, Rose-John S. Ciliary neurotrophic factor induces acute-phase protein expression in hepatocytes. FEBS Lett. 1992 Dec 21;314(3):280-4.
8. Graf U, Casanova EA, Cinelli P. The Role of the Leukemia Inhibitory Factor (LIF) - Pathway in Derivation and Maintenance of Murine Pluripotent Stem Cells. Genes (Basel). 2011 Mar 9;2(1):280-97.
9. Stewart CL, Kaspar P, Brunet LJ, Bhatt H, Gadi I, Köntgen F, Abbondanzo SJ. Blastocyst implantation depends on maternal expression of leukaemia inhibitory factor. Nature. 1992 Sep 3;359(6390):76-9.
10. Jorgensen MM, de la Puente P. Leukemia Inhibitory Factor: An Important Cytokine in Pathologies and Cancer. Biomolecules. 2022 Jan 27;12(2):217.
11. Fujio K, Komai T, Inoue M, Morita K, Okamura T, Yamamoto K. Revisiting the regulatory roles of the TGF-β family of cytokines. Autoimmun Rev. 2016 Sep;15(9):917-22.
12. Derynck R, Budi EH. Specificity, versatility, and control of TGF-β family signaling. Sci Signal. 2019 Feb 26;12(570):eaav5183.
13. Fabregat I, Moreno-Càceres J, Sánchez A, Dooley S, Dewidar B, Giannelli G, Ten Dijke P; IT-LIVER Consortium. TGF-β signaling and liver disease. FEBS J. 2016 Jun;283(12):2219-32.
14. Ferrari G, Cook BD, Terushkin V, Pintucci G, Mignatti P. Transforming growth factor-beta 1 (TGF-beta1) induces angiogenesis through vascular endothelial growth factor (VEGF)-mediated apoptosis. J Cell Physiol. 2009 May;219(2):449-58.
15. Wu M, Chen G, Li YP. TGF-β and BMP signaling in osteoblast, skeletal development, and bone formation, homeostasis and disease. Bone Res. 2016 Apr 26;4:16009.
16. LeRoith D, Roberts CT Jr. Insulin-like growth factors. Ann N Y Acad Sci. 1993 Aug 27;692:1-9.
17. Lin S, Zhang Q, Shao X, Zhang T, Xue C, Shi S, Zhao D, Lin Y. IGF-1 promotes angiogenesis in endothelial cells/adipose-derived stem cells co-culture system with activation of PI3K/Akt signal pathway. Cell Prolif. 2017 Dec;50(6):e12390.
18. Mairet-Coello G, Tury A, DiCicco-Bloom E. Insulin-like growth factor-1 promotes G(1)/S cell cycle progression through bidirectional regulation of cyclins and cyclin-dependent kinase inhibitors via the phosphatidylinositol 3-kinase/Akt pathway in developing rat cerebral cortex. J Neurosci. 2009 Jan 21;29(3):775-88.
19. Takahashi T, Ueno H, Shibuya M. VEGF activates protein kinase C-dependent, but Ras-independent Raf-MEK-MAP kinase pathway for DNA synthesis in primary endothelial cells. Oncogene. 1999 Apr 1;18(13):2221-30.
20. Nakatsu MN, Sainson RC, Pérez-del-Pulgar S, Aoto JN, Aitkenhead M, Taylor KL, Carpenter PM, Hughes CC. VEGF(121) and VEGF(165) regulate blood vessel diameter through vascular endothelial growth factor receptor 2 in an in vitro angiogenesis model. Lab Invest. 2003 Dec;83(12):1873-85.