BFP mRNA — Quality-Verified Blue Fluorescent Reporter
BFP mRNA – A Bright Reporter for Multicolor Fluorescence Imaging
BFP mRNA in multicolor fluorescence experiments
Multicolor fluorescence imaging is widely used to study complex biological processes. By combining fluorescent proteins with distinct emission spectra, researchers can track multiple proteins or cellular events simultaneously.
Because BFP occupies the blue spectral range, it serves as a complementary reporter to commonly used fluorescent proteins such as GFP, EGFP, and mCherry.
BFP mRNA (Blue Fluorescent Protein mRNA) enables clear blue fluorescence (~443 nm) for visualizing gene expression, protein localization, and live-cell processes. It is well suited for transient expression applications, including cell labeling, tracking, and multiplex imaging. Croyez offers both unmodified and modified BFP mRNA. The modified version is produced by in vitro transcription with a Cap 1 structure, poly(A) tail, and optimized nucleotides to enhance stability and reduce innate immune responses. All electrophoretic and capillary electrophoresis data shown below are generated using the modified BFP mRNA.
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Figure1. BFP mRNA (unmodified) was analyzed on a 2% E-Gel. Marker : Croyez RNA ladder (cat no: CR00004) |
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Figure 2. 100 ng RNA 65℃ loading 6 µL to 1% TAE agarose gel, 120 V 25 min |
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Figure 3. Capillary Electrophoresis BFP mRNA (unmodified) was analyzed by capillary electrophoresis. |
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Why Choose BFP mRNA?
・Useful for Complex Biological Models : BFP expression has been successfully used in advanced cell and organism models to enable enhanced microscopy when combined with GFP/RFP markers.