High Range RNA Ladder
The high range RNA Ladder, comprising nine single-stranded RNAs (0.2 k, 0.5 k, 1 k, 2 k, 3 k, 4 k, 6 k, 9 k, and 12 k ), is synthetically generated through in vitro transcription from a blend of nine linear DNA templates. This ladder serves as an effective tool for determining the size of single-stranded RNAs in native agarose gel electrophoresis. Visualization can be achieved using UV light post-ethidium bromide staining or nucleic acid safety dye staining. Notably, it acts as a reliable ssRNA size standard on native agarose gels.
✔️ Exclusive RNA ladders with an extensive range of up to 12,000 nt
✔️ Engineered for reliability and precision
Custom Service
→ Custom RNA Ladder
✔️ Exclusive RNA ladders with an extensive range of up to 12,000 nt
✔️ Engineered for reliability and precision
Custom Service
→ Custom RNA Ladder
Quality Control Testing
The banding pattern of RNA ladder on １% TAE or TBE agarose gels shows clear identifiable bands at each fragment, when stained with nucleic acid safety dye under UV light.
Storage Buffer
1 mM sodium citrate buffer (pH 6.4)
Storage & Stability
This product is stable for 5 months after storage at -80°C and should be avoided from repeated freeze/thaw cycles.
Handling Instruction
For optimal storage, aliquot the reagent into smaller quantities and store at recommended temperature.
Please promptly retrieve the required portion and return it to the appropriate storage temperature.
Recommended to Use
Ladder preparation for Electrophoresis
1. Mix 1 volume of RNA ladder with 3 volumes of 2X RNA Loading Dye.
2. Incubate at 65°C for 15 minutes.
3. Immediately place it on ice for 1-2 minutes
4. Load 0.5 μL of the prepared ladder for every mm of gel lane width (e.g., 4 μL for an 8 mm lane).
Applications
Electrophoresis
Note
1. To avoid ribonuclease contamination, using RNase-free water is necessary for sample dilution.
2. We do not recommend use of these markers as a quantitative standard.
Shipping Conditions
Dry ice
The banding pattern of RNA ladder on １% TAE or TBE agarose gels shows clear identifiable bands at each fragment, when stained with nucleic acid safety dye under UV light.
Storage Buffer
1 mM sodium citrate buffer (pH 6.4)
Storage & Stability
This product is stable for 5 months after storage at -80°C and should be avoided from repeated freeze/thaw cycles.
Handling Instruction
For optimal storage, aliquot the reagent into smaller quantities and store at recommended temperature.
Please promptly retrieve the required portion and return it to the appropriate storage temperature.
Recommended to Use
Ladder preparation for Electrophoresis
1. Mix 1 volume of RNA ladder with 3 volumes of 2X RNA Loading Dye.
2. Incubate at 65°C for 15 minutes.
3. Immediately place it on ice for 1-2 minutes
4. Load 0.5 μL of the prepared ladder for every mm of gel lane width (e.g., 4 μL for an 8 mm lane).
Applications
Electrophoresis
Note
1. To avoid ribonuclease contamination, using RNase-free water is necessary for sample dilution.
2. We do not recommend use of these markers as a quantitative standard.
Shipping Conditions
Dry ice
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The High Range RNA ladder contains various ssRNA sizes up to 12,000 nt, suitable for use on a 1%TAE agarose gel, with different amounts required per lane.
Capillary Electrophoresis
High-Resolution capillary electrophoresis separation of RNA Ladder.
The data demonstrates the production of RNA ladders with precise fragment sizes ranging from 0.2kb to 12kb.
The High Range RNA Ladder exhibits remarkable stability across a range of temperatures, including 4°C, -20°C, and -70°C
