SUMO Protease (ULP1) ELISA Kit
SUMO Protease (ULP1, Ubiquitin-like-specific-protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitin-like (UBL) protein, and SUMO from fusion proteins. Therefore, detection of ULP1 Protease is important for evaluating the level of ULP1 Protease in samples.
Croyez® SUMO Protease (ULP1) ELISA kit is an enzyme linked immunosorbent assay (ELISA) for the quantitative detection of ULP1 Protease level in cleavage sample solution. The SUMO Protease (ULP1) ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Croyez® SUMO Protease (ULP1) ELISA kit is an enzyme linked immunosorbent assay (ELISA) for the quantitative detection of ULP1 Protease level in cleavage sample solution. The SUMO Protease (ULP1) ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Method:
Sandwich ELISA
Principle of Assay:
SUMO Protease (ULP1) ELISA kit is used to detect ULP1 Protease in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-ULP1 Protease monoclonal antibody to the solid phase. ULP1 Protease in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-ULP1 Protease monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of ULP1 Protease level is accomplished by comparing the absorbance with standard curve.
Platform:
Pre-coated 96 Microplate (12 x 8 well strips)
Assay Time:
2 hours
Sensitivity:
0.0135 ng/ml
Detection Range:
1.5625 -100 ng/mL
Reactivity:
ULP1 Protease
Conjugation:
HRP
Storage:
Store at 2-8°C.
Alternate Names:
Small Ubiquitin-like Modifier Protease, ULP, Ubiquitin like protease, Ubiquitin-homology domain protein PIC1, Ubl-specific protease 1
Components:
For Research Use Only
Shipping Conditions:
Blue ice
Background Information:
SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitin-like (UBL) protein, and SUMO from fusion proteins. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. ULP1 protease has a His-tag for easy removal from a cleavage reaction by using nickel affinity resins. Notably, the cleavage reactions are available in a buffer containing 2 M urea.
Sandwich ELISA
Principle of Assay:
SUMO Protease (ULP1) ELISA kit is used to detect ULP1 Protease in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-ULP1 Protease monoclonal antibody to the solid phase. ULP1 Protease in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-ULP1 Protease monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of ULP1 Protease level is accomplished by comparing the absorbance with standard curve.
Platform:
Pre-coated 96 Microplate (12 x 8 well strips)
Assay Time:
2 hours
Sensitivity:
0.0135 ng/ml
Detection Range:
1.5625 -100 ng/mL
Reactivity:
ULP1 Protease
Conjugation:
HRP
Storage:
Store at 2-8°C.
Alternate Names:
Small Ubiquitin-like Modifier Protease, ULP, Ubiquitin like protease, Ubiquitin-homology domain protein PIC1, Ubl-specific protease 1
Components:
- SUMO Protease (ULP1) ELISA plate
- Standard
- Standard reconstitution buffer
- Standard & Sample diluent buffer
- HRP-antibody conjugate
- HRP-antibody conjugated diluent buffer
- 20 X wash buffer
- TMB
- Stop solution
- Microplate sealing film
For Research Use Only
Shipping Conditions:
Blue ice
Background Information:
SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitin-like (UBL) protein, and SUMO from fusion proteins. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. ULP1 protease has a His-tag for easy removal from a cleavage reaction by using nickel affinity resins. Notably, the cleavage reactions are available in a buffer containing 2 M urea.
Representative Standard Curve using SUMO Protease (ULP1) ELISA Kit.
