Sequence
MPPSGLRLLPLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESA
EPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSN
RLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQ
SSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQ
ALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the N-terminus

UnitProt ID
P01137
 
Source
HEK293

Endotoxin Test
<0.1 EU per 1 μg of the protein by the LAL method.

Activity
Measure by its ability to inhibit the IL-4 dependent proliferation in TF-1 cells. The ED₅₀ for this effect is < 0.1 ng/mL.
 
Purity
>95% as determined by SDS-PAGE. 

Form
Lyophilized

Storage Buffer
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.0.

Reconstitution
It is recommended to reconstitute the lyophilized protein in 10 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Stability & Storage
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.

Shipping Conditions
Blue ice