Customer Feedback-GFP mRNA-LNP Reporter Expression in Cancer Cell Models
Customer Feedback
GFP mRNA-LNP Shows Reporter Expression in Challenging Cancer Cell Models
Researchers working with disease-relevant cancer cell models often face challenges when using conventional DNA plasmid transfection. Some cell models may show weak reporter expression, inconsistent results, or require repeated optimization before a usable signal can be detected.In recent customer feedback, GFP mRNA-LNP was used to evaluate RNA delivery and reporter expression in multiple challenging cancer cell models, including cell lines related to T-cell acute lymphoblastic leukemia, triple-negative breast cancer, and glioblastoma / glioma research. Visible GFP reporter expression was observed by fluorescence microscopy after GFP mRNA-LNP treatment in the submitted customer data.
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The tested cell models included KE-37, a T-cell leukemia / T-ALL suspension cell model; BT-549, HCC1143, HCC1395, and MDA-MB-231, which are breast cancer or triple-negative breast cancer-related models; and LN229, T98G, and U87, which are glioblastoma or glioma-related models.
Data1.
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| BT-549 cells (2.5 × 10⁴ cells per well, 24-well plate) were transfected with 250 ng of GFP LNP-mRNA for 24 hours. | BT-549 cells (2.5 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA for 48 hours. |
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| HCC1143 cells (3.5 × 10⁴ cells per well, 24-well plate) were transfected with 225 ng of GFP LNP-mRNA and incubated for 24 hours. | HCC1143 cells (3.5 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 24 hours. |
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HCC1395 cells (4 × 10⁴ cells per well, 24-well plate) were transfected with 250 ng of GFP LNP-mRNA and incubated for 6 hours. |
HCC1395 cells (4 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 48 hours. |
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MDA-MB-231 cells (3 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 6 hours. |
MDA-MB-231 cells (3 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 72 hours. |
Data 2.
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KE-37 cells (5 × 10⁵ cells per well) were transfected with 200 ng of GFP LNP-mRNA and incubated for 3 days. |
Data 3.
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LN229 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 100 ng and incubated for 24 hours. |
LN229 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 200 ng and incubated for 24 hours. |
LN229 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 200 ng and incubated for 72 hours. |
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T98G cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 200 ng and incubated for 72 hours. |
T98G cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 250 ng and incubated for 48 hours. |
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U87 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 150 ng and incubated for 24 hours. |
U87 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 150 ng and incubated for 48 hours. |
Compared with DNA plasmid transfection, mRNA-LNP provides a more direct route to transient protein expression. DNA plasmids must enter the nucleus before transcription can occur, which may become a limitation in hard-to-transfect cells. In contrast, mRNA does not require nuclear entry. Once delivered into the cytoplasm, mRNA can be directly translated into protein.
Based on this customer feedback, GFP mRNA-LNP may serve as a practical reporter-based starting point for evaluating RNA delivery feasibility before moving to functional mRNA payloads such as Cas9 mRNA, Cre mRNA, ABEmax mRNA, PEmax mRNA, or other research applications.
Note: The customer feedback was based on fluorescence microscopy observation. Flow cytometry, image-based quantification, and cell viability assays were not included. Therefore, the results should be interpreted as visual reporter expression feedback, not quantitative transfection efficiency or cytotoxicity data.
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